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无色素及少色素性恶性黑色素瘤(简称恶黑),我们应用组织化学染色,S-100蛋白免疫组化染色及电镜检查,探讨对恶黑诊断和鉴别诊断的价值,并将22例恶黑(10例无色素性,12例少色素性恶黑)检查结果报告如下。材料及方法本科1957～1984年活检存档旧蜡块,用10%福尔马林固定,将石蜡切成片,厚5微米。一、组织化学法:①Gomori 网状纤维染色。②普鲁氏兰铁反应。③过氧化氢褪色素试验。④Masson Fontana 黑色素银染色。
For non-pigmented and less pigmented malignant melanoma (abbreviated as EH), we used histochemical staining, S-100 protein immunohistochemical staining, and electron microscopy to investigate the value of diagnosing and differential diagnosis of malignant black and 22 cases of malignant black (10 non-pigmented and 12 hypochromic black) test results are reported below. Materials and Methods Undergraduate biopsy files from 1957 to 1984 were archived in old wax blocks, fixed in 10% formalin, paraffin cut into slices and 5 μm thick. First, histochemical method: 1 Gomori reticular fiber staining. 2 Prussian blue iron reaction. 3 Hydrogen peroxide fading test. 4Masson Fontana melanin silver staining.