论文部分内容阅读
目的:探讨小鼠孕期酒精暴露对仔鼠齿状回苔藓细胞的影响,并进一步观察神经鞘磷脂合成酶2基因敲除(sphingomyelin synthase 2 knockout,SMS2-/-)与孕期酒精暴露和苔藓细胞丢失之间的关系。方法:采用PCR法对各分组仔鼠进行鉴定,利用SMS2-/-小鼠建立孕期酒精暴露模型,收集P14仔鼠,进行水迷宫实验,观察仔鼠寻台时间;然后利用免疫荧光染色法观察各分组仔鼠齿状回苔藓细胞数量的变化,免疫印迹技术检测P14仔鼠海马组织GluR2/3蛋白的相对表达量。结果:(1)与对照组相比,酒精组仔鼠的寻台时间相对较长(P<0.05),而SMS2-/-组仔鼠的寻台时间与野生组无明显的差异(P>0.05);(2)酒精组仔鼠齿状回GluR2/3的阳性细胞数明显少于对照组(P<0.05),且SMS2基因敲除后,与野生组仔鼠相比,SMS2-/-组仔鼠齿状回GluR2/3的阳性细胞数无明显差异(P>0.05)。结论:(1)酒精可诱导齿状回苔藓细胞的丢失,且降低GluR2/3的阳性表达;(2)SMS2基因的敲除对酒精诱导苔藓细胞丢失的作用相对较小;(3)苔藓细胞的丢失在一定程度上降低近期记忆功能,酒精可促进记忆功能的恶化。
OBJECTIVE: To investigate the effects of alcohol exposure during pregnancy on the gerbil moss cells of the gerbil, and further observe the effects of sphingomyelin synthase 2 knockout (SMS2 - / -) and alcohol exposure and moss cell loss during pregnancy The relationship between. Methods: The offspring rats were identified by PCR method. The model of alcohol exposure during pregnancy was established by SMS2 - / - mice. P14 pups were collected and subjected to water maze test to observe the time of seeking new rats. Then the immunofluorescence staining The number of gerbil moss cells in each group was changed. The relative expression of GluR2 / 3 protein in hippocampus of P14 pups was detected by Western blotting. Results: (1) Compared with the control group, the locomotion time of the offspring of alcoholic group was relatively longer (P <0.05), while that of the SMS2 - / - group was not significantly different from that of the wild group (P> 0.05). (2) Compared with the control group, the number of GluR2 / 3 positive cells in the dentate gyrus in the alcohol group was significantly lower than that in the control group (P <0.05) There was no significant difference in the number of positive cells of GluR2 / 3 in rat pups (P> 0.05). Conclusion: (1) Alcohol can induce the loss of dentate gyrus cells and decrease the positive expression of GluR2 / 3. (2) The effect of knockdown of SMS2 gene on alcohol-induced loss of moss cells is relatively small. (3) The loss of a certain extent reduce the recent memory function, alcohol can promote the deterioration of memory function.