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目的:探讨双氢青蒿素(DHA)对小鼠Lewis肺癌移植瘤的抑瘤效应及其作用机制。方法:C57BL/6J小鼠皮下接种3LL细胞(2×106)50只,随机分为5组,分别为生理盐水组、阳性对照顺铂(DDP)组和DHA高、中、低剂量组,检测各组小鼠的体质量变化和抑瘤率,应用流式细胞术进行瘤细胞的DNA倍体分析。结果:生理盐水组小鼠体质量比DHA高、中剂量增加明显,DHA低剂量抑瘤率为7.01%,较生理盐水组差异无统计学意义(t=0.872,P=0.395),中高剂量组抑瘤率分别为53.50%及59.24%,与生理盐水组比较差异有统计学意义(t=8.541,P=0.000;t=13.566,P=0.000),但抑瘤率均低于DDP组的92.36%,差异有统计学意义(t=-6.871,P=0.000;t=-9.667,P=0.000)。流式细胞术检测DHA能诱导肿瘤细胞凋亡,并能影响肿瘤的细胞周期,G0/G1期及G2/M期细胞数大量减少,细胞被阻滞在S1期,与生理盐水组比较,差异均有统计学意义,P值均为0.000。结论:在一定剂量范围内,口服DHA对Lewis小鼠肺癌有明显的抑制作用,可促进肿瘤细胞凋亡。
Objective: To investigate the anti-tumor effect of dihydroartemisinin (DHA) on Lewis lung carcinoma in mice and its mechanism. Methods: C57BL / 6J mice were subcutaneously inoculated with 3LL cells (2 × 106) 50 and randomly divided into 5 groups: normal saline group, DDP group and high, medium and low DHA groups Body mass changes and tumor inhibition rate in each group of mice, the application of flow cytometry DNA ploidy analysis of tumor cells. Results: Compared with DHA group, the body weight of the mice in the saline group was significantly higher than that in the DHA group, and the dose of DHA was 7.01%, which was significantly lower than that of the normal saline group (t = 0.872, P = 0.395) The tumor inhibition rates were 53.50% and 59.24%, respectively, which were significantly lower than those in the normal saline group (t = 8.541, P = 0.000; t = 13.566, P = 0.000) %, The difference was statistically significant (t = -6.871, P = 0.000; t = -9.667, P = 0.000). Flow cytometry detected DHA can induce tumor cell apoptosis, and can affect the tumor cell cycle, G0 / G1 phase and G2 / M phase a significant reduction in cell number, cells were arrested in the S1 phase, compared with the saline group, the difference All were statistically significant, P values were 0.000. Conclusion: Oral administration of DHA can significantly inhibit the growth of Lewis lung carcinoma in a dose range and promote the apoptosis of tumor cells.