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在大鼠背部皮下注入巴豆油,诱发无菌性炎症(AI)发热,致炎后第一天动物体温上升达高峰,第二天体温开始回降,在第八天体温回降至正常。实验分四组,第一组为对照组,不做致炎处理,第二组为发热高峰组,第三组为发热恢复组,观察大鼠AI发热高峰期及恢复期时不同脑区cAMP含量和腺苷酸环化酶(AC)活性的变化。第四组为发热恢复正常组.观察致炎后第一天至第八天动物体温从高峰障至正常的变化曲线,不做脑组织取材。结果发现:大鼠在发热高峰期与对照组比较,丘脑下部cAMP含量明显增加(P<0.01),并与体温变化呈正相关关系(r=0.893);AC活性显著增强(P<0.001),也与体温变化呈正相关关系(r=0.824),脑皮质、脑干cAMP含量与AC活性均无明显变化。发热恢复组与对照组比较:丘脑下部cAMP含量明显增加(P<0.05),丘脑下部、脑干AC活性均显著增强(P<0.05),而脑皮质cAMP含量和AC活性均无明显变化。作者推论:大鼠AI发热很可能是由于丘脑下部AC活性增强使局部cAMP含量增多所致。
Subcutaneous injection of croton oil in the back of the rats induced aseptic inflammation (AI) fever, the first day after the inflammation of animals rose to the peak body temperature, the next day the body temperature began to fall back to the normal body temperature on the eighth day. The experimental group was divided into four groups. The first group was the control group. The second group was fever peak group, and the third group was fever recovery group. The cAMP content in different brain regions during the peak fever and convalescence of rats And adenylyl cyclase (AC) activity. The fourth group was normal fever group.Observe the animal body temperature from peak to normal on the first day to the eighth day after inflammation, without making brain tissue. The results showed that the content of cAMP in the hypothalamus was significantly increased (P <0.01), and was positively correlated with the body temperature (r = 0.893), AC activity was significantly increased (P <0.001) in the peak fever in rats compared with the control group There was a positive correlation between body temperature and temperature (r = 0.824). No significant changes were observed in cAMP content and AC activity in cerebral cortex and brainstem. Compared with the control group, the levels of cAMP in the hypothalamus were significantly increased (P <0.05) and the AC activity in the hypothalamus and brainstem was significantly increased (P <0.05), while the cAMP content and AC activity in the cortex did not change significantly. The authors conclude that fever in rat AI is most likely due to increased cAMP content in the hypothalamus due to increased AC activity.