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报道甲地孕酮酶标免疫测定和兔血药浓度测定。抗血清特异性:除与甲孕酮、雌三醇交叉反应分别为29.6％和1.9％外,其余5种甾体均小于0.1％。灵敏度为0.1ng;回收率:0.5、1和2ng分别为99.5、80和86.8％;批内CV<10％,批间CV<20％。静脉注射和口服后兔血浆浓度分别是160和62ng/mL。静脉注射和口服后T1/2分别为15±3h和14±4h。生物利用度为64％。虽然酶标免疫测定(Enzyme Immunoassay),EIA)的灵敏度不及放射免疫测定(RIA),但本法无同位素污染,不需特殊设备和专门实验室,适合于基层医疗单位使用。
Reported megestrol enzyme immunoassay and rabbit plasma concentration determination. Antiserum specificity: Except cross-reaction with medroxyprogesterone and estriol 29.6% and 1.9%, the other five steroids were all less than 0.1%. The sensitivity was 0.1ng. The recoveries were 0.5, 1 and 2 ng, 99.5, 80 and 86.8% respectively. Intra-assay CV <10% and inter-assay CV <20%. Rabbit plasma concentrations after intravenous and oral administration were 160 and 62 ng / mL, respectively. T1 / 2 after intravenous and oral administration were 15 ± 3 h and 14 ± 4 h, respectively. Bioavailability was 64%. Although Enzyme Immunoassay (EIA) is less sensitive than radioimmunoassay (RIA), this method is isotopically-contaminated and does not require special equipment and specialized laboratories for use in primary care units.