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①目的研究不同饲养环境对鸡新纹状体(MNH)神经细胞树突棘的影响。②方法选择刚孵出的WhiteLeghorn鸡20只,随机分为两组,对照组同笼群养,实验组隔离饲养,7d后,其脑神经细胞发育基本成熟,将其处死并灌注固定,采用5%LuciferYelow对MNH神经细胞进行细胞内染色,并用共聚焦激光扫描显微镜进行扫描观察,对神经细胞树突棘进行分类、测量和统计分析。③结果实验组中各种树突棘的茎径平均值较对照组大(t=5.956,P<0.001);且实验组中S型和M型树突棘茎径与对照组比较,差异均有极显著性(t=4.25,5.11,P<0.01),T型树突棘长度也较对照组大(t=4.26,P<0.01)。④结论社会接触和相互学习能影响脑MNH神经细胞初期的发育成长,提高其兴奋性突触后电位,提高突触间的传递效率,并能促使M型、S型树突棘向T型转化
Objective To study the effects of different feeding environments on dendritic spines of neuron in chicken new striatum (MNH). Methods Twenty newly hatched WhiteLeghorn chickens were selected and randomly divided into two groups. The control group was housed in cage group. The experimental group was kept in isolation. After 7 days, the brain neurons developed basically mature. After being sacrificed and fixed by perfusion, % LuciferYelow MNH neurons intracellular staining, and confocal laser scanning microscopy scanning observations, nerve cell dendritic spine classification, measurement and statistical analysis. Results The average stem diameter of various dendritic spines in the experimental group was larger than that in the control group (t = 5.956, P <0.001). In the experimental group, (T = 4.25,5.11, P <0.01). The length of T-type dendritic spines was also significantly higher than that of the control group (t = 4.26, P <0.01) . ④Conclusion Social contact and mutual learning can affect the initial growth and development of MNH neurons in the brain, increase their excitatory postsynaptic potentials, increase the transfer efficiency between synapses, and promote the transformation of M and S dendritic spines into T type