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取紫菀(Aster tataricus)干燥根,以甲醇提取,提取液经二氯甲烷和正丁醇处理后,正丁醇可溶部分经Diaion HP-20柱层析,以水一甲醇溶液梯度(1:0～0:1)洗脱。80%甲醇洗脱部分再经硅胶柱层析,用二氯甲烷-甲醇溶液梯度(1:0～0:1)洗脱。10％甲醇洗脱部分重结晶后,置于ODS HPLC柱,以乙腈-水和甲醇-水溶液洗脱,得无色针状物。根据光谱数据的分析和Astin C的化学位移,确定
The dried roots of Aster tataricus were extracted with methanol and the extract was treated with methylene chloride and n-butanol. The n-butanol-soluble fractions were subjected to Diaion HP-20 column chromatography with a gradient of water to methanol (1:0). ~ 0:1) elution. The 80% methanol fraction was further subjected to silica gel column chromatography and eluted with a gradient of dichloromethane-methanol (1:0 to 0:1). The 10% methanol fraction was recrystallized and placed on an ODS HPLC column eluting with acetonitrile-water and methanol-water to give colorless needles. According to the analysis of spectral data and the chemical shift of Astin C, determine