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目的:探讨阿托伐他汀对兔急性心肌梗死后梗死心肌组织miR-221/miR-222表达以及血管新生的影响。方法:40只新西兰大白兔随机选取8只设为假手术组,只在左冠状动脉前降支下穿线不结扎,剩余32只兔建立急性心肌梗死模型后随机均分为2组:模型组和他汀组。他汀组给予阿托伐他汀钙片1mg·kg-1·d-1,连续灌胃4周;假手术组和模型组给予等量助溶剂连续灌胃4周。4周后处死,取梗死心肌组织,采用免疫组织化学法检测梗死区心肌组织微血管密度(MVD),采用RT-PCR法检测miR-221/miR-222的表达水平。结果:与模型组MVD(3.450±1.036)相比,他汀组(7.640±1.804)明显增加(P<0.05)。与假手术组miR-221/miR-222表达水平(0.716±0.083/0.692±0.069)比较,模型组miR-221/miR-222表达水平(1.010±0.043/1.009±0.042)明显升高,他汀组(0.431±0.111/0.374±0.118)明显降低(P<0.05)。他汀组梗死组织MVD和miR-221/miR-222的表达水平呈显著负相关(r=-0.755,P<0.01;r=-0.804,P<0.01)。结论:阿托伐他汀可能通过抑制miR-221/miR-222的表达促进心肌梗死后梗死心肌组织的血管新生。
Objective: To investigate the effect of atorvastatin on the expression of miR-221 / miR-222 and angiogenesis in infarcted myocardium in rabbits with acute myocardial infarction. Methods: Eight New Zealand white rabbits were randomly divided into sham-operated group, non-ligature only in the left anterior descending coronary artery, and the remaining 32 rabbits were randomly divided into 2 groups: model group and Statin group. Atorvastatin calcium tablets (1 mg · kg-1 · d-1) were given to the statin group for 4 weeks. The rats in the sham-operation group and the model group were given gavage for 4 weeks. After 4 weeks, the infarcted myocardium was removed and the myocardial microvessel density (MVD) was measured by immunohistochemistry. The expression of miR-221 / miR-222 was detected by RT-PCR. Results: Statin group (7.640 ± 1.804) increased significantly (P <0.05) compared with model group MVD (3.450 ± 1.036). The miR-221 / miR-222 expression level (1.010 ± 0.043 / 1.009 ± 0.042) in model group was significantly higher than that in sham operation group (0.716 ± 0.083 / 0.692 ± 0.069) (0.431 ± 0.111 / 0.374 ± 0.118) (P <0.05). The expression of MVD and miR-221 / miR-222 in the infarcted tissue of statin group was significantly negatively correlated (r = -0.755, P <0.01; r = -0.804, P <0.01). Conclusions Atorvastatin may promote angiogenesis in myocardial infarction after myocardial infarction by inhibiting the expression of miR-221 / miR-222.