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本文报道用异硫氰酸荧光素标记的花生凝集素(FTTC-PNA)来测定经小牛胸腺激肽诱导后小鼠胸腺细胞表面PNA受体的变化。小牛胸腺激肽与胸腺细胞在完全RPMI-1640培养液中,置37℃温育20小时,随后洗涤细胞两次,加FITC-PNA,置0～4℃保温2小时,再洗涤以除去未结合的FITC-PNA,用半乳糖与胸腺细胞上的FITC-PNA结合并使之洗脱下来,最后用MPF-4荧光分光光度计测定荧光度,以表示结合在细胞PNA受体上FITC-PNA的量,从而反映PNA受体的变化情况。我们研究了胸腺激肽与胸腺细胞的温育时间、不同剂量及酸、碱、热和酶处理后的胸腺激肽对PNA受体的影响。且比较了小牛脾、肾制剂和胸腺激肽的生物活性。实验结果得出经胸腺激肽温育后的胸腺细胞,其表面FITC-PNA的结合量要比对照组降低16～19％。
This article reports the use of fluorescein isothiocyanate-labeled peanut lectin (FTTC-PNA) to measure changes in the surface of the mouse thymocyte PNA receptor induced by calf thymus kinase. Calf thymus kinase and thymocytes were incubated in complete RPMI-1640 medium for 20 hours at 37 ° C. Cells were then washed twice with FITC-PNA and incubated at 0-4 ° C for 2 hours before being washed to remove The bound FITC-PNA was conjugated to and eluted with FITC-PNA on thymocytes using galactose and finally the fluorescence was measured using a MPF-4 fluorescence spectrophotometer to indicate the binding of FITC-PNA to cellular PNA receptors Of the amount of PNA receptor to reflect the changes. We studied the incubation time of thymulin and thymus cells, and the effect of different doses of thymus kinins on PNA receptors after acid, alkali, heat and enzyme treatment. The biological activities of calf spleen, kidney preparation and thymus kinin were compared. The results showed that thymus-stimulated thymus cells incubated on the surface of the FITC-PNA binding capacity than the control group decreased by 16 to 19%.