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目的:揭示水通道蛋白3(AQP3)在维吾尔族妇女宫颈癌发生、浸润过程中的表达及意义。方法:应用RT-PCR、Westernblot和免疫荧光染色检测AQP3在82例宫颈癌、36例宫颈上皮内瘤样病变Ⅲ级和42例正常宫颈中的表达。结果:在正常宫颈和宫颈鳞癌组织中AQP3 mRNA和蛋白均表达,蛋白水平表达有差异(P<0.05),而mRNA表达无差异(P>0.05)。AQP3在正常宫颈、宫颈上皮内瘤样病变Ⅲ级和宫颈癌中的阳性率分别为19.05%、27.78%和42.68%,阳性表达率存在显著性差异(P<0.05)。结合临床病理资料分析,AQP3阳性表达率与宫颈癌患者临床分期、肿瘤直径、浸润深度呈正相关(P<0.05),与宫颈癌大体分型、病理分级和淋巴结转移无关(P>0.05)。结论:AQP3在子宫颈鳞癌疾病发生、肿瘤生长和局部浸润过程中可能发挥重要的生理作用。
Objective: To reveal the expression and significance of aquaporin-3 (AQP3) in Uighur women with cervical carcinogenesis and infiltration. Methods: The expression of AQP3 in 82 cases of cervical cancer, 36 cases of cervical intraepithelial neoplasia grade Ⅲ and 42 cases of normal cervix was detected by RT-PCR, Western blot and immunofluorescence staining. Results: AQP3 mRNA and protein were expressed in normal cervical and cervical squamous cell carcinoma tissues, but the expression of AQP3 protein was different (P <0.05), but there was no difference in mRNA expression (P> 0.05). The positive rates of AQP3 in normal cervical and cervical intraepithelial neoplasia grade Ⅲ and cervical cancer were 19.05%, 27.78% and 42.68%, respectively. The positive expression rate of AQP3 was significantly different (P <0.05). Combined with the clinicopathological data, the positive expression rate of AQP3 was positively correlated with the clinical stage, tumor diameter and depth of invasion of cervical cancer (P <0.05), but not with the general classification of cervical cancer, pathological grade and lymph node metastasis (P> 0.05). Conclusion: AQP3 may play an important physiological role in the development of cervical squamous cell carcinoma, tumor growth and local infiltration.