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采用普通高效液相色谱法,紫外检测分离氨基酸具有快速、简便、灵敏、准确及经济等特点,避免了柱前、柱后衍生方法的复杂操作和特殊装置。通过小儿血浆中游离苯丙氨酸的测定可发现小儿痴呆症,及早通过药物治疗及控制饮食可收到良好的效果。本工作具有重要的社会效益。用所建立的方法对近400名儿童进行了普查,测得血浆中游离苯丙氨酸含量范围(3.0～18.4)μg/ml;资料报道,国内用磺基水杨酸法测得
The separation of amino acids by ordinary high performance liquid chromatography and UV detection is fast, simple, sensitive, accurate and economical, and avoids the complicated operation and special devices of pre-column and post-column derivatization methods. Through the determination of free phenylalanine in children plasma can be found in children with dementia, as early as possible through the drug treatment and diet can receive good results. This work has important social benefits. The established method for screening nearly 400 children were measured plasma levels of free phenylalanine (3.0 ~ 18.4) μg / ml; data reported that the domestic sulfosalicylic acid method was measured