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Objective To investigate expression of inositol 1,4,5 trisphosphate receptor (IP-3R) mRNA on sacroplasmic reticular in myocardium of spontaneous hypertension rats (SHRs) and cultured vascular smooth muscle cells (VSMC) of rats and effects of perindopril and urapidil on them. Methods SHRs were orally given perindopril (1.0 mg·kg+{ 1}·d+{ 1}) or urapidil (15 mg·kg+{ 1}·d+{ 1}) for 24 weeks, respectively. Expression of IP-3R mRNA was examined by semi quantitative reverse transcription polymers chain reaction (RT PCR) using three oligonuclotide primers for each subtype of IP-3R with β actin as internal label. Results All subtypes of IP-3R were expressed in myocardium of SHR, WKY and cultured VSMC. Expression of IP-3R mRNA in left ventricle of SHR was markedly enhanced. Urapidil could down regulate expression of IP-3R Ⅰand IP-3R Ⅲ, perindopril slightly increased expression of IP-3R Ⅱ and decreased expression of IP-3R Ⅰand IP-3R Ⅲ in myocardium of SHR. Conclusion Our results suggest that expression of IP-3R mRNA in cardiovascular system could be regulated by urapidil and perindopril.
Objective To investigate expression of inositol 1,4,5 trisphosphate receptor (IP - 3R) mRNA on sacroplasmic reticular in myocardium of spontaneous hypertension rats (SHRs) and cultured vascular smooth muscle cells (VSMC) of rats and effects of perindopril and urapidil on them. Methods SHRs were orally given perindopril (1.0 mg · kg + {1 } · d + {1 }) or urapidil (15 mg · kg Expression of IP - 3R mRNA was examined by semi quantitative reverse transcription polymers chain reaction (RT PCR) using {1 } · d + {1 }} for 24 weeks, Results of All subtypes of IP - 3R were expressed in myocardium of SHR, WKY and cultured VSMC. Expression of IP - 3R mRNA In the left ventricle of SHR was markedly enhanced. Urapidil could down regulate expression of IP - 3R I and IP - 3R III, perindopril slightly increased expression of IP - 3RII and decreased expression of IP - 3R Ⅰand IP - 3RIII in myocardium of SHR. Conclusion Our results suggest that expression of IP - 3R mRNA in cardiovascular system could be regulated by urapidil and perindopril.