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[Objective] The aim was to determine the puerarin and daidzin contents in total flavonoids extracts of RADIX PUERARIAE. [Method] HPLC conditions were: chromatographic column of C18 column, mobile phase of acetonitrile-water (25∶75, V/V), detection wavelength of 250 nm, flow speed of 0.8 ml/min, column temperature of room temperature and sampling amount of 4 μl. [Results] Within the determination range, puerarin and daidzin control substances showed good linear relationship. And The puerarin and daiazin contents in total flavonoids of RADIX PUERARIAE were 5.41 and 7.50 μg/ml, respectively. [Conclusion] This research provided basis for better utilization of RADIX PUERARIAE.
[Objective] The aim was to determine the puerarin and daidzin contents in total flavonoids extracts of RADIX PUERARIAE. [Method] HPLC conditions were: chromatographic column of C18 column, mobile phase of acetonitrile-water (25:75, V / V) Detection wavelength of 250 nm, flow rate of 0.8 ml / min, column temperature of room temperature and sampling amount of 4 μl. [Results] Within the determination range, puerarin and daidzin control substances showed good linear relationship. And The puerarin and daiazin contents in total flavonoids of RADIX PUERARIAE were 5.41 and 7.50 μg / ml, respectively. [Conclusion] This research provides basis for better utilization of RADIX PUERARIAE.