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血红蛋白可同化学致癌物和突变原化合,这种烷基化作用提供了一种剂量监测方法。其作用机理是,它们与细胞大分子中的亲核点形成共价键。作者测定了15种致癌物和致突变物与大鼠血红蛋白化合的情况。此项研究中用雄性Sprague-Dawley大鼠,重200～250 g。使用C~(14)放射性同位素标记物。所用15种化学致癌物是:甲基·甲磺酸盐,二甲基亚硝酸胺,N-亚硝酸甲基脲,苯并(a)芘,甲基-N′-硝基-N-亚硝基奎尼定,3-甲基胆蒽,亚硝基二乙胺,苯,2-乙酰胺基芴,7,12-二甲基苯并蒽,黄曲霉毒素B_1,联苯胺,氯仿,四氯化碳。给大鼠灌胃实验试剂(1 ml/kg体重),乙醚麻醉后,用肝磷脂化的注射器作心脏穿刺,收集血液(24
Hemoglobin can be combined with chemical carcinogens and mutants, and this alkylation provides a means of dose monitoring. Its mechanism of action is that they form covalent bonds with nucleophilic sites in cellular macromolecules. The authors determined the presence of 15 carcinogens and mutagens with hemoglobin in rats. Male Sprague-Dawley rats in this study, weighing 200-250 g. C ~ (14) radioactive isotope labels were used. The 15 chemical carcinogens used were: methyl methanesulfonate, dimethylnitrosamine, N-nitrilomethylurea, benzo (a) pyrene, methyl-N’-nitro-N- Nitroquinidine, 3-methylcholanthrene, nitrosodiethylamine, benzene, 2-acetamidofluorene, 7,12-dimethylbenzanthracene, aflatoxin B 1, benzidine, chloroform, Carbon tetrachloride. The rats were gavaged with experimental reagent (1 ml / kg body weight), anesthetized with ether, and then punctured with heparinized syringes to collect blood (24