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目的:探讨大黄对大鼠胃排空运动的影响及其机制,为临床应用大黄提供理论依据。方法:32只Wistar大鼠随机分为大黄组及对照组,分别给大鼠灌服大黄水提液或蒸馏水1h、6h后,以葡聚糖蓝-2000为胃肠内标记物观察大鼠胃排空的变化,同时用免疫组化染色观察胃窦肌间神经丛P物质(SP)、血管活性肠肽(VIP)的分布变化。结果:灌服大黄水提液后,大鼠胃排空运动显著抑制(P<0.05或P<0.01),胃窦肌间神经丛SP的分布明显减少(P<0.05或P<0.01),VIP分布明显增加(P<0.05或P<0.01)。结论:大黄对大鼠胃运动有明显的抑制作用,其机制可能与胃窦肌间神经丛SP的分布减少及VIP的分布增加有关。
Objective: To investigate the effect of rhubarb on gastric emptying in rats and its mechanism, so as to provide a theoretical basis for the clinical application of rhubarb. Methods: Thirty-two Wistar rats were randomly divided into the rhubarb group and the control group. Rats were orally treated with rhubarb aqueous extract or distilled water for 1 h and 6 h respectively. Glucan blue-2000 was used as the intragastric marker to observe the rat stomach. The change of emptying and the distribution of substance P (SP) and vasoactive intestinal peptide (VIP) in the antrum myenteric plexus were observed by immunohistochemical staining. RESULTS: After emptying rhubarb aqueous extract, rats’ gastric emptying movement was significantly inhibited (P<0.05 or P<0.01), and the distribution of SP in myenteric plexus of gastric antrum was significantly reduced (P<0.05 or P<0.01). The distribution was significantly increased (P<0.05 or P<0.01). Conclusion: Rhubarb has a significant inhibitory effect on gastric motility in rats, and its mechanism may be related to the decreased distribution of SP in the antrum myenteric plexus and increased VIP distribution.