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目的 探索特定基因操纵区CpG岛高甲基化对人类造血系统恶性肿瘤的作用。方法 利用甲基化特异性聚合酶链反应 (MSP)的方法 ,用亚硫酸氢钠修饰后的DNA为模板进行甲基化特异性聚合酶链反应 (PCR) ,测定了 2 5例急性髓系白血病 (AML) ,15例慢性粒细胞白血病 (CML) ,16例骨髓增生异常综合征 (MDS)和 12例多发性骨髓瘤 (MM)患者P1 5INK4B基因在操纵区 5′ CpG岛异常甲基化的发生率。结果 P1 5INK4B基因异常甲基化的发生率 :AML为 84% ,CML为 0 ,MDS为 5 0 % ,MM为 75 %。高危型的MDS较低危型更易发生甲基化。在MM中 ,P1 5INK4B甲基化一般发生在早期 ,与骨髓象的幼稚程度关系密切。结论 调节细胞生长和分化的P1 5INK4B基因高甲基化是使P1 5INK4B 基因失活的主要原因之一 ,CpG岛高甲基化与恶性血液病的发生密切相关。
Objective To explore the effect of hypermethylation of CpG island in specific gene manipulation region on human hematopoietic malignancies. Methods Methylation-specific polymerase chain reaction (PCR) was performed using methylation-specific polymerase chain reaction (MSP) and DNA modified by sodium bisulfite as template. Twenty-five acute myeloid The aberrant methylation of 5 ’CpG island in the control region of P1 5INK4B gene in leukemia (AML), 15 cases of chronic myeloid leukemia (CML), 16 cases of myelodysplastic syndrome (MDS) and 12 cases of multiple myeloma (MM) The incidence of. Results The incidence of abnormal methylation of P1 5INK4B gene was 84% in AML, 0 in CML, 50% in MDS and 75% in MM. Lower risk of high-risk type of MDS more susceptible to methylation. In MM, P1 5INK4B methylation usually occurs in the early stage, closely related to the immature level of bone marrow. Conclusion The hypermethylation of P1 5INK4B gene, which regulates cell growth and differentiation, is one of the main reasons for the inactivation of P1 5INK4B gene. The hypermethylation of CpG island is closely related to the occurrence of hematologic malignancies.