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体外受精过程中胚胎发生污染比较少见,一旦发生常导致胚胎的死亡,造成体外受精-胚胎移植(IVF- ET)失败。我院共行IVF-ET 152周期,发现2例污染,报告如下。例1患者27岁,结婚4年,曾两次因胚胎停止发育行人工流产,子宫输卵管造影术(HSG)提示双侧输卵管阻塞,于2003年2月在本院生殖中心行IVF- ET治疗。采用长方案促超排卵,共获卵母细胞9个,置四孔皿中培养4～6h后加入已获能上游的精子悬液。16～18h后,去除颗粒细胞,观察受精情况,8个受精形成双原核,1个三原核,移入生长液。随后在倒置显微镜下检查四孔皿内精子活动情况,发现孔中存在杆状微生物,疑胚胎有污染。随即对剩余的洗卵液、受精液、上游精液进行检查,并进行细
In vitro fertilization embryo contamination is relatively rare, in the event of death often lead to embryos, resulting in IVF-ET failure. IVF-ET 152 cycles of our hospital, found two cases of pollution, the report is as follows. Example 1 The patient was 27 years old and married for 4 years. Her abortion was stopped twice because of embryonic arrest. Hysterosalpingography (HSG) prompted bilateral tubal occlusion. IVF-ET was given to our reproductive center in February 2003. Using a long-term program to promote ovulation, a total of 9 oocytes were placed in a four-hole dish cultured 4 ~ 6h after the sperm suspension has been able to gain access to the upper. After 16-18h, the granulosa cells were removed and the fertilization was observed. Eight fertilizers formed the dihydroprogenetic nucleus and one triplastic nucleus and transferred into the growth fluid. Subsequent examination of the sperm motility in a four-well dish under an inverted microscope revealed the presence of rod-shaped microorganisms in the well and suspected contamination of the embryo. Then the remaining oviposit, semen, upstream semen were checked and fine