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连续注射1wk眼镜蛇神经毒素后,用放射免疫测定法测定对照组及神经毒素组大鼠5个脑区亮啡肽样免疫反应物质及甲硫啡肽样免疫反应物质的含量。神经毒素组两种脑啡肽样物质在下丘脑、中脑+丘脑两个脑区的含量变化均出现明显升高(p<0.05),海马及后脑的含量均无显著变化。吗啡在很低浓度对低频电刺激豚鼠回肠及小鼠输精管收缩有明显抑制作用。神经毒素的存在与否并不影响吗啡的效应。结果提示,神经毒素可能并不直接与吗啡受体产生相互作用,但其镇痛机理可能涉及中枢内源性阿片肽能系统。
After continuous injection of 1-wk cobra neurotoxin, the content of leu-enkephalin-like immunoreactive substance and methionine-like immunoreactive substance in 5 brain regions of rats in control group and neurotoxin group were determined by radioimmunoassay. Neurotropin group, two enkephalin peptide-like substances in the hypothalamus, midbrain + thalamus in two brain regions were significantly increased (p <0.05), hippocampus and hindbrain content did not change significantly. Morphine significantly inhibited the vas deferens contraction in guinea pig ileum and mice at low frequency. The presence of neurotoxin does not affect the effect of morphine. The results suggest that neurotoxins may not directly interact with morphine receptors, but its analgesic mechanism may involve the central endogenous opioid peptide system.