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寻找有效的抗恶性疟原虫混合基因疫苗 ,探讨其在宿主体内的免疫反应。将恶性疟原虫重组质粒 pc DNA 3-EBA175 / HRP 经骨骼肌途径单独注射或与有性期阶段的重组质粒 pc DNA3- Pfs2 5混合注射免疫 Balb/ c小鼠。对小鼠的骨骼肌进行预处理 ,即于注射前 7d在左后肢股四头肌注射 0 .5 %盐酸布比卡因 5 0μl,注射深度为 2 m m。观察免疫后不同时间点小鼠血清 Ig G抗体滴度、脾淋巴细胞增殖反应、CD4+ / CD8+ T细胞亚群比率和 NK细胞杀伤活性的变化。结果显示 ,pc DNA3- EBA 175 / HRP 单独肌肉注射或与 pc DNA3- Pfs2 5混合肌肉注射免疫小鼠 ,均可见血清 Ig G抗体滴度增高、经恶性疟原虫抗原刺激后的特异性 T淋巴细胞增殖反应增强、CD4+ / CD8+ T细胞比率下降以及 NK细胞杀伤活性增强 ;加强免疫注射机体的免疫反应能增强。提示肌肉注射 DNA疫苗为一有效的免疫途径 ,采用编码恶性疟原虫两个基因的重组质粒单独免疫或与编码不同阶段基因的重组质粒混合免疫小鼠 ,均能诱导明显的体液免疫反应、细胞免疫和 NK细胞杀伤活性
Looking for effective anti-Plasmodium falciparum mixed gene vaccine to explore its immune response in the host. Plasmodium falciparum recombinant plasmid pc DNA 3-EBA175 / HRP was injected into the Balb / c mice via skeletal muscle alone or in combination with the recombinant plasmid pcDNA3-Pfs2 5 during sexual stage. Skeletal muscle of mice was pretreated, that is, 5 μl of 0.5% bupivacaine hydrochloride was injected into the quadriceps femoris of the left hind limb 7 days before injection, and the injection depth was 2 μm. The changes of serum IgG antibody titers, splenic lymphocyte proliferation, CD4 + / CD8 + T cell subsets and NK cytotoxicity were observed at different time points after immunization. The results showed that the titers of serum Ig G antibody were increased when pc DNA3-EBA 175 / HRP was intramuscularly injected or intramuscularly immunized with pc DNA3-Pfs2 5, and the specific T lymphocytes stimulated by P. falciparum antigen Enhanced proliferative response, decreased CD4 + / CD8 + T cell ratio and enhanced cytotoxicity of NK cells; enhanced immune response can be enhanced. Suggesting that intramuscular injection of DNA vaccine is an effective immunization pathway. The recombinant plasmids encoding two genes of Plasmodium falciparum alone or in combination with recombinant plasmids encoding different stages of the gene could induce significant humoral immune response, cellular immunity And NK cell killing activity