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旨在研究化学改性的甘蔗渣作为固定化载体对丙酮丁醇梭菌Clostridium acetobutylicum XY16发酵制备生物丁醇的影响。首先利用不同浓度的聚乙烯亚胺(PEI)和1 g/L戊二醛(GA)对甘蔗渣表面进行化学改性,增强甘蔗渣对Clostridium acetobutylicum XY16的附载能力。经4 g/L聚乙烯亚胺和1 g/L戊二醛改性的甘蔗渣(添加量10 g/L)应用到固定化批次发酵中,发酵36 h后丁醇和总溶剂浓度最高,分别达到了12.24 g/L和21.67 g/L,同时溶剂的生产速率达到0.60 g/(L·h),生产速率比游离细胞和未改性甘蔗渣固定化细胞分批发酵分别提高了130.8%和66.7%。在此基础上对改性甘蔗渣固定化的细胞进行6次重复批次发酵,丁醇和总溶剂的产量稳定,溶剂生产速率逐渐提高至0.83 g/(L·h),同时转化率也提高至0.42 g/g。
The aim of this study was to investigate the effect of chemically modified bagasse as an immobilized carrier on biobutanol production by Clostridium acetobutylicum XY16 fermentation. Firstly, the surface of bagasse was chemically modified with polyethyleneimine (PEI) and 1 g / L glutaraldehyde (GA) to enhance the ability of bagasse to support Clostridium acetobutylicum XY16. The bagasse modified with 4 g / L polyethylenimine and 1 g / L glutaraldehyde (dosage 10 g / L) was applied to the immobilized batch fermentation. Butanol and total solvent concentrations were the highest after 36 h of fermentation, Respectively, reached 12.24 g / L and 21.67 g / L respectively, and the production rate of the solvent reached 0.60 g / (L · h). The production rate was increased by 130.8% compared with the batch fermentation of immobilized cells of free cells and unmodified bagasse, And 66.7%. On this basis, the modified bagasse immobilized cells were fermented in 6 replicates, the yield of butanol and total solvent was stable, the solvent production rate was gradually increased to 0.83 g / (L · h), and the conversion rate was also increased to 0.42 g / g.