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目的建立乙醇现场固定口腔脱落细胞进行微核试验的方法。方法利用无水乙醇现场固定口腔脱落细胞,保存0～30h后进行微核试验与常规口腔脱落细胞微核试验、外周血淋巴细胞微核试验进行比较。结果乙醇现场固定法的口腔脱落细胞在4、8、24h后的微核率与常规口腔脱落细胞微核率和外周淋巴细胞法微核率(Oh)差异无统计学意义(P>0.05),而常规法口腔脱落细胞在保存4h后则出现微核率下降,与外周血培养法的微核率差异有统计学意义(P<0.05)。结论无水乙醇现场固定口腔脱落细胞能够有效延长保存时间,微核试验结果稳定,适合用于现场采样。
Objective To establish a method for micronucleus test of fixed-oral exfoliated cells in ethanol. Methods Decellularized cells were fixed on the site with absolute ethanol. The micronucleus test was performed after stored for 0 ~ 30 h, compared with micronucleus test of oral exfoliated cells and micronucleus test of peripheral blood lymphocytes. Results There was no significant difference in micronucleus rate of oral exfoliated cells after 4, 8 and 24 hours of ethanol fixation compared with that of conventional oral exfoliated cells and peripheral lymphocyte micronuclei (Oh) (P> 0.05) However, the rate of micronuclei in normal oral exfoliated cells decreased 4h after storage, which was significantly different from that in peripheral blood culture (P <0.05). Conclusion The immobilized oral exfoliated cells can effectively prolong the storage time, and the results of micronucleus test are stable. It is suitable for on-site sampling.