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目的:探讨凋亡相关蛋白激酶1(DAPK1)、端粒酶催化亚单位(hTERT)在口腔鳞状细胞癌(OSCC)中的表达,并探讨其临床意义。方法:应用免疫组织化学方法检测DAPK1、hTERT在93例OSCC组织及10例癌旁正常组织中的表达,并分析其与OSCC临床病理参数之间的关系及其在OSCC浸润前沿中的作用。结果:癌旁正常组织中DAPK1的表达显著高于OSCC组织中DAPK1的表达,且高、中、低分化OSCC组织中DAPK1的表达比较均有显著差异(P<0.05),OSCC浸润前沿组织中DAPK1的表达低于非前沿部分(P<0.05),而DAPK1的表达与OSCC浸润前沿的IFG总分无显著相关性(P>0.05)。癌旁正常组织中hTERT的表达显著低于OSCC组织中hTERT的表达(P<0.05),且高、中、低分化OSCC组织中hTERT的表达比较均有显著差异(P<0.05);OSCC浸润前沿组织中hTERT的表达高于非前沿部分(P<0.05),且与OSCC浸润前沿的IFG总分有关(P<0.05)。DAPK1、hTERT的表达均与OSCC患者的性别、年龄、淋巴结转移无显著相关性(P>0.05)。结论:口腔鳞状细胞癌组织,特别是其前沿组织中DAPK1的表达显著下调和hTERT的表达明显上调,可能通过阻碍口腔鳞状细胞癌细胞凋亡,共同促进口腔鳞状细胞癌的生长、分化和浸润。
Objective: To investigate the expression of apoptosis-associated protein kinase 1 (DAPK1) and telomerase catalytic subunit (hTERT) in oral squamous cell carcinoma (OSCC) and to explore its clinical significance. Methods: The expressions of DAPK1 and hTERT in 93 OSCC tissues and 10 adjacent normal tissues were detected by immunohistochemistry. The relationship between them and clinicopathological parameters of OSCC and their role in the infiltration of OSCC were analyzed. Results: The expression of DAPK1 in normal paracancerous tissues was significantly higher than that in OSCC tissues, and the expression of DAPK1 in high, moderate and poorly differentiated OSCC tissues was significantly different (P <0.05). The expression of DAPK1 (P <0.05). However, there was no significant correlation between the expression of DAPK1 and the total score of IFG in the frontal infiltration of OSCC (P> 0.05). The expression of hTERT in normal paracancer tissues was significantly lower than that in OSCC tissues (P <0.05), and the expression of hTERT in high, moderate and poorly differentiated OSCC tissues was significantly different (P <0.05). The frontal invasion of OSCC The expression of hTERT in the tissue was higher than that in the non-frontal part (P <0.05), and was related to the IFG score of the frontal OSCC (P <0.05). There was no significant correlation between the expression of DAPK1 and hTERT and sex, age and lymph node metastasis in patients with OSCC (P> 0.05). Conclusion: The down-regulation of DAPK1 and the up-regulation of hTERT in oral squamous cell carcinoma tissues, especially in the frontal tissues, may promote the growth and differentiation of oral squamous cell carcinoma by blocking the apoptosis of oral squamous cell carcinoma cells, And infiltration.