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应用Ｓａｎｇｅｒ的双脱氧链终止法，对本室构建的人类白细胞介素２基因重组子ｐＲＥＴ－９０的４４０ｂｐｃＤＮＡ功能片段（ＮｃｏＩ克隆点的ＨｇｉＡＩ－ＤｒａＩ片段）进行ＤＮＡ碱基序列分析。结果表明：白细胞介素２ｃＤＮＡ功能片段按照正确的方向和翻译顺序连接在载体ｐＥＴ３ｄ的ＮｃｏＩ－ＢａｍＨＩ切点之间。
The nucleotide sequence of the 440bp cDNA fragment (HgiAI-DraI fragment of NcoI cloning site) of human interleukin 2 gene recombinant pRET-90 constructed by Sanger was analyzed by DNA sequencing using Sanger’s dideoxy chain termination method. The results showed that the functional fragment of interleukin 2 cDNA was ligated between the NcoI-BamHI cleavage sites of the vector pET3d in the correct orientation and translation order.