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目的:探讨广西肝癌高发区HLA-DRB1*08/16等位基因与广西原发性肝细胞肝癌(hepatocellular carcinoma,HCC)家族聚集性的关系.方法:在广西肝癌高发区配对选择肝癌高发家族成员、无癌家族成员各200例作为研究对象.应用聚合酶链反应/序列特异性引物(polymerase chain reaction/sequence specific p r i m e r,P C R-S S P)检测研究对象中H L ADRB1*08/16等位基因的频率,分析其与广西肝癌高发区肝癌家族聚集性的关系.结果:(1)HLA-DRB1*08等位基因在肝癌高发家族成员组携带率(6.0%)及无癌家族成员组的携带率(5.5%)经比较差别无统计学意义(2=0.046,P=0.830),而HLA-DRB1*16等位基因在肝癌高发家族成员组携带率(24%)明显高于无癌家族成员组(15.5%),该基因在两组中分布有显著差异(2=4.559,P=0.033);(2)随着高发家族中肝癌患者人数的增加,HLA-DRB1*08等位基因的表达频率无差别(5.4%vs 6.5%,2=0.120,P=0.729),而HLA-DRB1*16等位基因的表达频率明显升高(17.2%vs 29.9%,2=4.401,P=0.036).结论:HLA-DRB1*08可能与广西肝癌家族聚集无明显关系,HLA-DRB1*16可能与广西肝癌家族聚集有关.
Objective: To investigate the relationship between HLA-DRB1 * 08/16 alleles and familial aggregation of hepatocellular carcinoma (HCC) in high incidence area of hepatocellular carcinoma in Guangxi.Methods: The high incidence of HCC in Guangxi , 200 cancer-free family members were selected as study subjects.The frequency of HL ADRB1 * 08/16 alleles in the study subjects was detected by polymerase chain reaction / sequence specific primer (PC RS SP) (1) The prevalence of HLA-DRB1 * 08 alleles in the high incidence of HCC family members (6.0%) and in the non-cancer family members ( 5.5%) had no significant difference (2 = 0.046, P = 0.830). However, the HLA-DRB1 * 16 allele was significantly higher in the HCC patients (24%) than in the non-cancer family members (2 = 4.559, P = 0.033). (2) The frequency of HLA-DRB1 * 08 allele was no significant difference with the increase of the number of HCC patients (5.4% vs 6.5%, 2 = 0.120, P = 0.729), whereas the HLA-DRB1 * 16 allele (17.2% vs 29.9%, 2 = 4.401, P = 0.036) .Conclusion: HLA-DRB1 * 08 may not be related to familial aggregation of hepatocellular carcinoma in Guangxi. HLA-DRB1 * 16 may be related to familial aggregation of hepatocellular carcinoma in Guangxi .