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经超数排卵处理的成年大鼠,其排卵量受年龄的影响。正常的成年大鼠中,经超数排卵处理后,直径250～549μm的非闭锁性卵泡都有可能排卵,而大于549μm的卵泡则在动情前即可随LH峰而排卵。在以前的报道中,并未阐明大鼠超数排卵的能力与卵泡大小和年龄的关系。本实验的目的在于确定2～78周龄大鼠卵巢内250～549μm和大于549μm的非闭锁性卵泡数量的变化。材料和方法本实验采用220只2～78周龄Wistar-Imamichi系处女大鼠。饲养在24±2℃条件下,每昼夜照明14小时(上午7时开灯)。每天上午10时左右采检阴道涂片,判断其动情周期。选用至少连续两次4天动情
Adult ovariectomized rats, their ovulation by age. Normal adult rats, after superovulation treatment, non-blocking follicles of 250 ~ 549μm in diameter are likely to ovulate, and follicles greater than 549μm before ovulation with LH peak estrus. In previous reports, the relationship between the ability of superovulation and follicular size and age in rats was not elucidated. The purpose of this experiment was to determine the change in the number of non-occlusive follicles between 250 and 549 μm and greater than 549 μm in the ovaries of 2-78-week-old rats. MATERIALS AND METHODS: Wistar-Imamichi male rats aged 2 to 78 weeks were used in this study. Feeding at 24 ± 2 ℃, lighting 14 hours a day and night (7:00 am lights). About 10 o’clock every day vaginal smear sampling to determine the cycle of estrus. Use at least two consecutive four days of passion