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乙酰胆碱酯酶(AChE)抑制剂是治疗阿尔茨海默病的一个重要策略。本研究对48种中药材的乙醇提取物和水提取物进行了体外AChE抑制活性的筛选,微板测试法结果表明28种中药材的乙醇提取物和11种中药材的水提取物具有AChE抑制活性。其中,香薷、益智仁、生地黄和荷叶乙醇提取物的AChE抑制活性最强,它们在终浓度为0.1mg/mL时的抑制活性分别为(68.63±1.12)%,(44.49±3.66)%,(43.78±4.76)%和(42.63±8.31)%。薄层色谱–生物自显影的方法亦证明了这四种中药材具有抑制AChE的活性成分。本实验结果为治疗阿尔茨海默病中药的传统应用提供了部分支持。
Acetylcholinesterase (AChE) inhibitors are an important strategy in the treatment of Alzheimer’s disease. In this study, 48 kinds of Chinese herbal medicines ethanol extract and water extract in vitro AChE inhibitory activity screening, microplate test results show that 28 kinds of Chinese herbal medicines and 11 kinds of Chinese herbal medicines water extract AChE inhibition active. Among them, the AChE inhibitory activity of the extract from Xiangpi, Yizhen, Rehmannia glutinosa and lotus leaf was the strongest, and the inhibitory activity against AChE was (68.63 ± 1.12)% and (44.49 ± 3.66)% respectively at the final concentration of 0.1mg / mL, (43.78 ± 4.76)% and (42.63 ± 8.31)%, respectively. Thin-layer chromatography-autobiography method also proved that these four kinds of Chinese herbal medicines have inhibitory activity of AChE. The results of this experiment provide some support for the traditional application of traditional Chinese medicine in the treatment of Alzheimer’s disease.