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淋巴细胞微核出现率是电离辐射生物效应的敏感指标之一,除用于观察辐射效应外,还广泛用于研究化学诱变剂和某些疾病。为取得地区的正常值和增补国内资料,1983年6月至11月对贵阳地区正常人外周血淋巴细胞微核进行了调查。材料和方法受检者74人均系贵阳地区健康献血员,其中男30例,女44例,年龄范围18~48岁,平均29.6岁。微核标本的制作:取0.5毫升肝素抗凝的静脉血,用甲基纤维素浓集分离法,提取淋巴
Lymphocyte micronucleus incidence is one of the sensitive indicators of biological effects of ionizing radiation, in addition to the observation of radiation effects, but also widely used in the study of chemical mutagens and certain diseases. In order to obtain the normal value of the area and supplement the domestic data, the normal human peripheral blood lymphocyte micronucleus in Guiyang area were investigated from June to November, 1983. Materials and methods Subjects 74 per capita health Guiyang blood donors, including 30 males and 44 females, aged 18 to 48 years, mean 29.6 years. Preparation of micronucleus specimens: Take 0.5 ml heparin anticoagulant venous blood, with methyl cellulose concentration separation method, extraction of lymph