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有关延髓巨细胞网状核内突触小球的超微结构,迄今尚未见专文报道,为此,用健康成兔6只,经颈总动脉以生理盐水灌流冲洗,快速断头,迅速取下平闩以上水平的延髓(厚2mm),经5％戊二醛磷酸缓冲液作整体固定,解剖镜下取出延髓巨细胞网状核组织块,复经5％戊二醛及1％锇酸双固定,常规脱水,环氧树脂618包埋,LKB超薄切片机切片,厚约500A。醋酸铀和枸椽酸铅双染。H—600电镜下观察,结果如下: 家兔延髓巨细胞网状核内有大量轴突终末及树突。轴突终末的形状及大小很不一致,其内含有密集的突触小泡及线粒体,多数为突触前成分。树突干或树突棘有Ⅰ、Ⅱ两型。Ⅰ型不含突触小泡,Ⅱ型含少量突触小泡。轴突与树突间形成复杂的突触联系。
For the medulla oblongata giant cell mesh nucleus within the ultrastructure of synaptic spheres, so far no special reports, therefore, with healthy adult rabbit 6, the common carotid artery perfusion with saline irrigation, rapid decapitation, rapid removal of flat Above the level of the bulbar medulla oblongata (thickness 2mm), 5% glutaraldehyde phosphate buffer for the whole fixed dissection microscopic removal of giant cell medulla oblongata tissue block, complex by 5% glutaraldehyde and 1% osmium tetroxide dual fixation , Conventional dehydration, embedded in epoxy resin 618, sliced LKB ultra-thin slicer, about 500A thick. Uranyl acetate and lead citrate double dye. H-600 electron microscopy, the results are as follows: rabbit medulla oblongata giant cell mesh nucleus within a large number of axon terminals and dendrites. Axial terminal shape and size is very inconsistent, which contains dense synaptic vesicles and mitochondria, most of the pre-synaptic composition. Dendritic stem or dendritic spines are Ⅰ, Ⅱ two types. Type I does not contain synaptic vesicles, type II contains a small amount of synaptic vesicles. Axons and dendrites form complex synaptic connections.