细胞形态与DNA含量在乳腺良恶性病变的诊断价值

• 作 者: 师永红 宫丽平 孙慧宽 李增山
• 机 构: 内蒙古医学院病理教研室
• 出 处: 内蒙古医学院学报
• 发表日期: 1998年04期
• 关键词: 乳腺良恶性病变 DNA 计算机图像分析 回代 逐步判别分析 软件检测 乳腺癌转移 蒸馏水冲洗 腋淋巴结转移 淋巴转移

目的：研究乳腺良恶性病变细胞形态与ＤＮＡ含量。方法：利用传统的Ｆｅｕｌｇｅｎ染色及计算机图像分析系统软件检测细胞形态及ＤＮＡ含量。结果：ａ．乳腺良恶性病变之间、乳腺癌Ⅰ级与Ⅱ级、Ⅲ级之间、淋巴转移组与非转移组之间ＮＡ、ＣＡ、Ｎ／Ｐ及Ｐｎ有显著差别（Ｐ＜００１）；ｂ．乳腺良恶性病变之间ＤＮＡ含量存在显著差别Ｐ＜００１；ｃ．综合细胞形态及ＤＮＡ含量参数经逐步判别分析建立综合判别式，良性病变回代率为１００％，恶性肿瘤回代率９８７５％。结论：Ｎ／Ｐ，ＮＡ，ＣＡ及ＤＮＡ含量为乳腺良恶性病变提供可靠的差别依据，ＮＡ及Ｎ／Ｐ是判别乳腺癌分级和转移的重要因素。

Objective: To study the morphology and DNA content of benign and malignant breast lesions. METHODS: Cellular morphology and DNA content were detected using traditional Feulgen staining and computer image analysis system software. Result: a. There were significant differences in NA, CA, N/P, and Pn between benign and malignant breast lesions, between breast cancer grades I and II, and between lymphatic metastases and non-metastatic breast cancers (P<001); b . There was a significant difference in DNA content between benign and malignant breast lesions P<001; c. Comprehensive discriminant analysis was used to establish a comprehensive discriminant equation based on comprehensive cell morphology and DNA content parameters. The rate of benign lesions was 100%, and the rate of malignant tumors was 9875%. Conclusion: N/P, NA, CA and DNA content provide a reliable basis for differential diagnosis of benign and malignant breast lesions. NA and N/P are important factors in the classification and metastasis of breast cancer.