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本文研究了2-[(3.5-二溴-2-吡啶)偶氮]-5-氨基酚(简称3.5—Br_2—PAAP)与钴显色反应的适宜条件,其它离子的影响,确定了络合物的组成。实验表明,在 PH=4～5范围内钴与3.5—Br_2—PAAP 形成1:2的紫红色络合物.试剂的最大吸收峰为430nm,络合物的最大吸收峰为540nm,摩尔消光系数ε_(540)=6·48×10~4m01~(-1)cm~(-1),遵守比尔定律的范围为0～25μg/25ml。桑德尔灵敏度为0·00051微克 cm~(-2),表现稳定常数为3.6×10~6。通过对矿样、维生素 B_(12)针剂、小麦进行测定,取得了满意的结果。
In this paper, the suitable conditions for the chromogenic reaction of 2 - [(3.5-dibromo-2-pyridyl) azo] -5-aminophenol (referred to as 3.5-Br_2-PAAP) with cobalt were studied. Composition of things. Experiments show that cobalt and 3.5-Br_2-PAAP form a purple complex of 1: 2 in the range of pH = 4 ~ 5. The maximum absorption peak of the reagent is 430nm, the maximum absorption peak of the complex is 540nm, the molar extinction coefficient The ε_ (540) = 6.48 × 10 ~ 4m01 ~ (-1) cm ~ (-1), which obeys Beer’s law in the range of 0 ~ 25μg / 25ml. The Sensitivity of Sander was 0.00051 μg cm -2, and the stability constant was 3.6 × 10 -6. Through the determination of mineral sample, vitamin B_ (12) injection and wheat, satisfactory results were obtained.