论文部分内容阅读
目的 从正常垂体及垂体瘤组织中克隆新的全长cDNA。方法 垂体cDNA文库构建 ,大规模表达序列标签 (ESTs)产生及生物信息学处理 ,芯片拼接及cDNA末端快速扩增 (RACE)。结果 从正常垂体及垂体瘤组织分别克隆 41条和 2 6条新的全长cDNA ,共 6 7条。在这些基因中 ,与内分泌功能相关者 16条 ;至少有 2个是具有典型信号肽结构的分泌蛋白 ;3个是具有典型穿膜结构的膜蛋白。在 5个参与激素信号转导的新基因中 ,至少有 2个可能参与酪氨酸激酶型受体的信号转导过程。结论 通过ESTs手段 ,短期内克隆到 6 7条新的全长cDNA。在这些新基因中 ,有些可能在垂体的生理及病理过程中起重要作用。
Objective To clone new full-length cDNA from normal pituitary and pituitary tumors. Methods Pituitary cDNA library construction, generation of large-scale expressed sequence tags (ESTs) and bioinformatics processing, chip splicing and rapid amplification of cDNA ends (RACE). Results From the normal pituitary and pituitary tumor tissues, 41 and 26 new full-length cDNAs were cloned, totaling 67. Among these genes, there are 16 related to endocrine function; at least 2 are secretory proteins with typical signal peptide structure; 3 are membrane proteins with typical transmembrane structures. Of the five new genes involved in hormone signal transduction, at least two may be involved in the signal transduction process of tyrosine kinase type receptors. Conclusion By using ESTs, 67 new full-length cDNAs were cloned in short term. Among these new genes, some may play an important role in the physiological and pathological processes of the pituitary gland.