Simplified three-dimensional culture system for long-term expansion of embryonic stem cells

• 作 者: Christina McKee Mick Perez-Cru
• 机 构: OU-WBInstituteforStemCellandRegenerativeMedicine,DepartmentofBiologicalSciences,BeaumontHealthSystem
• 出 处: 世界干细胞杂志：英文版（电子版）
• 发表日期: 2015年7期
• 关键词: THREE-DIMENSIONAL CULTURE PLURIPOTENCY EMBRYONIC s Three-dimensional culture Plu
• 基金项目: Oakland University and Oakland University-William Beaumont Institute for Stem Cell and Regenerative Medicine(OU-WB ISCRM)

AIM: To devise a simplified and efficient method for long-term culture and maintenance of embryonic stem cells requiring less frequent passaging. METHODS: Mouse embryonic stem cells(ESCs) labeled with enhanced yellow fluorescent protein were cultured in t