人的真皮的 microvascular endothelial 房间的自发的不减

• 作 者: Laura DeBusk Suzanne Fernandez
• 机 构: DepartmentofRadiationOncology,DepartmentofUrologicSurgery,CenterforCancerResearch
• 出 处: 世界干细胞杂志：英文版（电子版）
• 发表日期: 2010年5期
• 关键词: SPONTANEOUS IMMORTALIZATION ANGIOGENESIS ENDOTHELIAL cell Spontaneous immortaliz
• 基金项目: Supported by (in part) Grants from NIH (CA108856, NS45888 and AR053718) to Lin,PC and training grants from NIH to DeBusk L (T32CA009592)

AIM:To establish and characterize a spontaneously immortalized human dermal microvascular endothelial cell line,iHDME1.METHODS:We developed a spontaneous immortalization method.This approach is based on the application of optimized culture media and cultu