分光光度法测定维他锌片中维生素B_2的含量

• 作 者: 姜心如 黄慧敏 孙亦平
• 机 构: 南京药学院药物分析教研室,南京药学院药物分析教研室,南京药学院药物分析教研室,
• 出 处: 南京药学院学报
• 发表日期: 1984年03期
• 关键词: 分光光度法测定 维生素B_2 测定标准 标准贮备液 变异系数 连二亚硫酸钠 吸收度 发色基团 异咯嗪 氢核

本文依据核黄素异咯嗪环上发色基团中活泼双氢,在连二亚硫酸钠作用下能还原为无色双氢核黄素的原理,采用分光光度法测定还原前后的吸收度,通过差值(即为核黄素的吸收度)求得该片中维生素B_2的含量。此法简便、专属性较好。测得结果经统计处理,平均回收率为98.87％,变异系数为0.42％,样品含量测定标准差为S_x=0.69,变异系数为0.83％。

Based on the principle of active dihydrogen in the chromophore of riboflavin alloxazine ring and reduction to colorless dihydrodipine under the action of sodium dithionite, the absorbance before and after reduction was determined by spectrophotometry Difference (that is, the absorption of riboflavin) obtained in the film of vitamin B_2 content. This method is simple, better specificity. The results obtained by statistical analysis, the average recovery was 98.87%, coefficient of variation of 0.42%, the standard deviation of the sample was Sx = 0.69, coefficient of variation was 0.83%.