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目的观察球形孢子丝菌由菌丝相向酵母相转换形态学的变化,在三种培养基的酵母转化率。方法将64株球形孢子丝菌于脑心浸液琼脂(BHI)35℃观察菌落及显微镜下酵母转化形态,并与0.5%葡萄糖脑心浸液琼脂(GSBHI)、马龄薯(PDA)培养基上酵母相转化率进行比较。结果多次传代能使酵母转化更完全,BHI转化率48.43%,GSBHI转化率65.63%,PDA转化率为20.31%。结论球形孢子丝菌由菌丝相至酵母相转化需经过连续多次传代;在GSBHI培养基中35℃酵母转化率最高。
OBJECTIVE To observe the morphological changes of C. sphaeroides transformated by mycelial phase to yeast phase and the yeast conversion rate in three media. Methods Sixty-four strains of Sporotrichia capsici were observed under colony and microscopy yeast transformation conditions at 35 ℃ of brain-heart infusion agar (BHI), and compared with 0.5% glucose brain heart infusion agar (GSBHI), potato medium The yeast phase conversion rates were compared. The results of multiple passages can make yeast transformation more complete, BHI conversion rate of 48.43%, GSBHI conversion rate of 65.63%, PDA conversion rate of 20.31%. Conclusion The transformation of mycelial phase to mycelial phase of Sphaerotheca fulgiformis requires multiple passages in succession. The transformation rate of yeast in GSBHI medium is the highest at 35 ℃.