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背景:巢蛋白是一种存在于神经干细胞的特异性抗原,在神经系统发生病变或损伤引起再生时广泛表达,因此巢蛋白表达常用作判定神经系统发生病变或损伤后能否促进神经再生的一种手段。目的:从神经再生和神经干细胞激活的角度,探讨外源性促红细胞生成素对新生鼠缺氧缺血性脑损伤后神经干细胞巢蛋白表达的影响。方法:结扎大鼠右侧颈总动脉和8%低氧暴露2h制备新生大鼠缺氧缺血性脑损伤模型。对照组仅游离右侧颈总动脉,不予结扎和缺氧处理。干预组大鼠缺氧缺血后立即腹腔注射重组人促红细胞生成素5000IU/kg,1次/d,连用3d。缺氧缺血性脑损伤组大鼠缺氧缺血后连续腹腔注射等量生理盐水溶液3d。每组随机取8只分别于术后4,7,14d处死。应用免疫组化方法和计算机图像分析技术检测不同时点海马齿状回巢蛋白标记阳性细胞的变化。结果与结论:各时点缺氧缺血性脑损伤组巢蛋白阳性细胞数较对照组增加(P<0.05);各时点干预组巢蛋白阳性细胞较对照组和缺氧缺血性脑损伤组均增加(P<0.05)。3组大鼠海马齿状回区巢蛋白阳性细胞数均于术后7d达高峰。结果提示早期给予重组人促红细胞生成素可促使新生鼠缺氧缺血性脑损伤后海马齿状回区巢蛋白表达增加,促进神经干细胞的增殖再生,在缺氧缺血性脑损伤后神经再生、修复中发挥一定的保护作用。
BACKGROUND: Nestin, a specific antigen present in neural stem cells, is expressed extensively when regeneration occurs due to pathological changes or damage in the nervous system. Therefore, nestin expression is often used as a marker for determining whether nerve regeneration or neurogenesis can be promoted Kind of means. OBJECTIVE: To investigate the effect of exogenous erythropoietin on the expression of nestin in neural stem cells of neonatal rats after hypoxic-ischemic brain damage from the perspective of nerve regeneration and neural stem cell activation. Methods: The hypoxic-ischemic brain damage model of neonatal rats was established by ligation of the right common carotid artery in rats and exposure to 8% hypoxia for 2 hours. The control group only free right common carotid artery, not ligation and hypoxia. Rats in the intervention group were injected intraperitoneally with recombinant human erythropoietin 5000 IU / kg once a day for hypoxia and ischemia for 3d consecutive days. Rats in hypoxic-ischemic brain damage group were injected intraperitoneally with normal saline solution for 3 days after hypoxia-ischemia. Eight rabbits in each group were sacrificed at 4, 7 and 14 days after operation respectively. Immunohistochemistry and computer image analysis were used to detect the changes of dentate gyrus protein positive cells in hippocampus at different time points. RESULTS AND CONCLUSION: The number of nestin-positive cells in hypoxic-ischemic brain injury groups at each time point was higher than that in control group (P <0.05). At each time point, the number of nestin-positive cells in intervention group was significantly higher than that in control group and hypoxic-ischemic brain injury Group were increased (P <0.05). The number of hippocampal dentate gyrus nestin positive cells in the three groups all reached the peak at 7 days after operation. The results suggest that the early administration of recombinant human erythropoietin can promote the expression of hippocampal dentate gyrus nestin in neonatal rats after hypoxic-ischemic brain damage and promote the proliferation and regeneration of neural stem cells in the regeneration of neurons after hypoxic-ischemic brain damage , Repair play a protective role.