论文部分内容阅读
目的: 测定和分析cDNA 克隆(CO111)与抗恶性疟原虫兔血清和1株单克隆抗体呈阳性反应的序列。方法:采用PCR扩增CO111 克隆的cDNA 片段,纯化后经Klenow 酶补平,与M13m p18 载体连接,转化到大肠杆菌(E.coli) JM109。PCR筛选和鉴定M13单链,PEABI373ADNA自动测序仪测序,PC/GENE和GenBank (EM-BL)等软件进行序列分析和比较。结果:CO111 cDNA克隆含有233对核苷酸对。与GenBank 数据库中恶性疟原虫DNA比较,未发现与该cDNA相同的序列。结论:分离出1 个新的与抗恶性疟原虫抗体呈阳性反应的抗原cDNA克隆
OBJECTIVE: To determine and analyze the sequence of cDNA clone (CO111) positive with the anti-Plasmodium falciparum rabbit serum and one monoclonal antibody. Methods: cDNA fragment of CO111 clone was amplified by PCR. After purification, the fragment was filled in by Klenow enzyme, ligated with M13m p18 vector and transformed into E. coli JM109. PCR screening and identification of M13 single chain, PEABI373ADNA automatic sequencing sequencer, PC / GENE and GenBank (EM-BL) and other software sequence analysis and comparison. RESULTS: The CO111 cDNA clone contained 233 pairs of nucleotide pairs. Compared with Plasmodium falciparum DNA in the GenBank database, the same sequence as the cDNA was not found. Conclusions: One new cDNA clone of the antigen that is positive for anti-Plasmodium falciparum antibodies was isolated