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本文旨在阐明接触氯丹后在神经递质γ-氨基丁酸(GABA)系统中出现的神经生化改变。实验动物取雄性CD-1小鼠(20～25 g)。氯丹溶解在玉米油中,给实验动物一次腹腔注射氯丹1 g/kg,对照组小鼠注射等量的玉米油。给氯丹8小时后,照Bernasconi氏方法测定GABA转化,具体步骤如下:给实验动物腹腔注GABA转氨酶抑制剂氨氧基乙酸(AOAA)20mg/kg。部分小鼠在给予AOAA后立即处死,作为0时对照;其余小鼠在给予AOAA 30分钟或60分钟后分别处死,在冰块上迅速取下鼠脑,分离脑组织,置于-80℃液氮中待用。用微量酶促荧光测定法测定GABA含量;用市售染料(Bio-Rad)比色法测定蛋白质含量,脑组织中GABA含量以μg/mg蛋白质表示。以给予AOAA后立即处死的小鼠脑中GABA含量作为基值,与给予AOAA 30分钟和60分
This article aims to elucidate the neurobiological changes that occur in the neurotransmitter gamma-aminobutyric acid (GABA) system after exposure to chlordane. Experimental animals were male CD-1 mice (20 ~ 25 g). Chlordane was dissolved in corn oil, and intraperitoneal injection of chlordane 1 g / kg was given to experimental animals. The control mice were injected with the same amount of corn oil. Eight hours after chlordane administration, the GABA transformation was determined by the Bernasconi method as follows: The experimental animals were injected intraperitoneally with GABA aminotransferase inhibitor aminoxyacetic acid (AOAA) 20 mg / kg. Some mice were sacrificed immediately after the AOAA administration as a control at time 0; the remaining mice were sacrificed 30 minutes or 60 minutes after the administration of AOAA respectively. The brain was quickly removed on ice and the brain tissue was separated and stored at -80 ° C. Nitrogen stand-by. The content of GABA was determined by micro-enzymatic fluorimetry; the protein content was determined by the commercially available dye (Bio-Rad) colorimetric method and the content of GABA in brain tissue was expressed as μg / mg of protein. The GABA content in the brains of mice immediately after the administration of AOAA was taken as a base value, compared with 30 minutes and 60 minutes for AOAA administration