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目的探讨昆仙胶囊对博来霉素诱导的肺间质纤维化小鼠肺组织病理和血清白细胞介素(IL)-6、IL-10、IL-13、粒-巨噬细胞集落刺激因子(GM-CSF)表达的影响。方法将60只C57BL/6小鼠按随机数字法分成空白组、模型组、昆仙胶囊组及甲泼尼龙组,每组15只。空白组气管内注入0.9%氯化钠注射液0.02 ml,其余3组以气管内注入博来霉素(5 mg/kg)建立肺纤维化模型,并于造模后第7、14、28天分批处死小鼠,留取血样测定细胞因子IL-6、IL-10、IL-13、GM-CSF的含量,并取肺脏采用苏木精-伊红(HE)及Masson染色法观察肺组织病理变化。结果 (1)与空白组对比,其他各组第7、14、28天小鼠体质量下降(P<0.05);与模型组对比,昆仙胶囊和甲泼尼龙组小鼠体质量有所增加(P<0.05);(2)在第7、14、28天昆仙胶囊组肺泡炎和肺纤维化程度明显轻于模型组,小鼠肺泡炎和肺纤维化病理评分降低(P<0.05);且第28天时,昆仙胶囊组仅形成轻中度肺间质纤维化。(3)在第7、14、28天昆仙胶囊组血清IL-6、IL-10、IL-13、GM-CSF较模型组明显降低(P<0.05),而昆仙胶囊组与甲泼尼龙组对比差异无统计学意义(P>0.05)。结论昆仙胶囊能显著降低肺间质纤维化小鼠血清IL-6、IL-10、IL-13、GM-CSF表达水平,减轻肺组织肺泡炎及肺纤维化程度,从而抑制博来霉素诱导的小鼠的炎症反应和肺间质纤维化的发生发展。
Objective To investigate the pathological changes of lung tissue and interleukin (IL)-6, IL-10, IL-13, granulocyte-macrophage colony-stimulating factor in mice induced by bleomycin-induced pulmonary interstitial fibrosis by Kunxian Capsules. The effect of GM-CSF) expression. Methods 60 C57BL/6 mice were randomly divided into blank group, model group, Kunxian capsule group and methylprednisolone group, 15 rats in each group. In the blank group, 0.02 ml of 0.9% sodium chloride injection was instilled into the trachea, and the other three groups were intubated with bleomycin (5 mg/kg) to establish a pulmonary fibrosis model, and on the 7th, 14th, and 28th days after modeling. Mice were sacrificed in batches and blood samples were taken to determine the levels of cytokines IL-6, IL-10, IL-13, and GM-CSF. Lungs were also examined by hematoxylin-eosin (HE) and Masson staining. Pathological changes. Results (1)Compared with the blank group, the body weight of mice in the other groups was decreased on the 7th, 14th, and 28th days (P<0.05). Compared with the model group, the body weight of mice in Kunxian capsule and methylprednisolone group increased. (P<0.05); (2) On the 7th, 14th and 28th days, the degree of alveolitis and pulmonary fibrosis in the Kunxian capsule group was significantly lighter than that of the model group, and the pathological scores of alveolitis and pulmonary fibrosis were reduced in the mice (P<0.05). On the 28th day, only mild to moderate pulmonary interstitial fibrosis was formed in the Kunxian capsule group. (3) On the 7th, 14th and 28th days, the serum IL-6, IL-10, IL-13 and GM-CSF in the Kunxian capsule group were significantly lower than those in the model group (P<0.05), while the Kunxian capsule group and Jiapian group No significant difference was found in the nylon group (P>0.05). Conclusion Kunxian capsule can significantly reduce the expression of IL-6, IL-10, IL-13 and GM-CSF in serum of mice with pulmonary interstitial fibrosis, reduce the degree of pulmonary alveolitis and pulmonary fibrosis, and thus inhibit bleomycin Induced inflammatory reactions in mice and development of pulmonary interstitial fibrosis.