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目的探讨高原习服过程中大鼠血浆瘦素(leptin)水平及弓状核内c-fos阳性神经元数目的变化。方法将48只健康成年SPF级雄性SD大鼠随机分为对照[西宁(海拔2 262 km)]组和第1、3、7、15、30天组[花石峡(海拔4 300 km)],每组8只。于饲养的既定时间检测血浆瘦素水平和弓状核中c-fos阳性神经元数目。结果与对照组比较,从急进高原第1天开始,各组大鼠血浆瘦素水平和下丘脑弓状核c-fos阳性神经元数目均升高,差异有统计学意义(P<0.01)。随着急进高原时间的延长,大鼠血浆瘦素水平和下丘脑弓状核c-fos阳性神经元数目均呈先上升后下降的趋势,且分别在第7、3天达到峰值。结论高原习服过程中大鼠血浆瘦素水平及弓状核内c-fos阳性神经元数目呈现先升高后降低的变化规律,推测血浆瘦素可能参与了高原习服不良的发生。
Objective To investigate the changes of plasma leptin and the number of c-fos-positive neurons in the arcuate nucleus during the plateau acclimatization. Methods 48 healthy adult SPF male SD rats were randomly divided into control (Xining (2 262 km) Each group of 8. Plasma leptin levels and the number of c-fos-positive neurons in the arcuate nucleus were measured at established times of feeding. Results Compared with the control group, the levels of plasma leptin and the number of c-fos positive neurons in the arcuate nucleus of the hypothalamus were significantly increased from the first day on to the plateau (P <0.01). The leptin level and the number of c-fos positive neurons in the arcuate nucleus of the hypothalamus tended to first increase and then decrease with the prolongation of the acute plateau, and reached the peak on the 7th and the 3rd days respectively. Conclusions Plasma leptin level and the number of c-fos positive neurons in the arcuate nucleus increase first and then decrease in the plateau acclimatization process. It is speculated that plasma leptin may be involved in the occurrence of poor acclimatization in the plateau.