Inhibition of haringtonine-induced apoptosis by tetradecanoylphorbol acetate in human leukemia HL-60

• 作 者: 孟凡宏 何琪杨 张鸿卿 薛绍白
• 机 构: 北京师范大学生物系
• 出 处: Acta Pharmacologica Sinica
• 发表日期: 1997年05期
• 关键词: 三尖杉酯碱类 细胞凋亡 HL-60 apoptosis by human in leukemia of 喜树碱

目的：研究十四酰佛波乙酸酯（ＴＡ）预处理后，三尖杉酯碱（Ｈａｒ）和喜树碱（Ｃａｍ）诱导人白血病ＨＬ６０细胞凋亡的变化．方法：染色质凝集观察，流式细胞术，ＤＮＡ琼脂糖凝胶电泳和点杂交．结果：ＴＡ２００ｎｍｏｌ·Ｌ－１预处理ＨＬ６０细胞６ｈ，明显抑制Ｈａｒ０１ｍｇ·Ｌ－１作用３ｈ诱导的细胞凋亡，但只部分抑制Ｃａｍ０２ｍｇ·Ｌ－１作用３ｈ诱导的细胞凋亡．ＴＡ预处理ＨＬ６０细胞明显降低ｃｍｙｃ基因的表达．结论：ＴＡ明显抑制由Ｈａｒ和部分抑制由Ｃａｍ诱导的ＨＬ６０细胞凋亡，而且ＴＡ预处理的细胞中ｃｍｙｃ基因表达明显下降，这可能导致了对诱导细胞凋亡的抑制．

OBJECTIVE: To study the changes of HL-60 cell apoptosis induced by harringtonine and camptothecin in human leukemia pretreated with tetradecanoyl phorbol acetate (TA). Methods: Chromatin agglutination, flow cytometry, DNA agarose gel electrophoresis and dot blotting. Results: TA 60 nmol·L-1 pretreated HL-60 cells for 6h significantly inhibited the apoptosis induced by Har01mg · L-1 for 3h, but only partially inhibited the apoptosis induced by Cam02mg · L-1 for 3h . TA pretreatment HL 60 cells significantly reduce cmyc gene expression. CONCLUSION: TA significantly inhibits the apoptosis of HL-60 cells induced by Cam and partly by Cam, and that the expression of c-myc gene in TA-pretreated cells is significantly decreased, which may lead to the inhibition of apoptosis induction.