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研究脑内交感抑制系统中中缝隐核（NRO）至中脑导水管周围灰质腹侧部（VPAG）通路的地茶酚胺递质定量。采用推挽灌流结合高效液相一电化学检测法观察连续电刺激NRO3Omin，vPAG中野上腺素（A）、去甲肾上腺素（NA）释放的变化。结果：刺激NRO10～30min，vPAG灌流液中A含量明显增加（尸＜0．O5）；刺激NRO10～20min，vPAG中NA含量也增加（P＜005）。在上述基础上，于刺激NRO前双侧侧脑室注射5－HT2受体拮抗剂ketanserin（100μg／10μl），给药后电刺激NRO10～3Omin内样品中A、NA含量无明显变化（P＞005），ketanserin阻断了刺激NRO引起的A、NA释放增加的效应。结论：NRO至vPAG投射的递质是ANA；5－HT能神经元参与NRO至vPAG的反馈效应，可能由5－HT2受体参与介导。
To investigate the quantitative determination of catecholamine neurotransmitter in the sympathetic repressor system of the central synovectomy (NRO) to the midbrain periaqueductal gray (VPAG) pathway in the brain. The changes of the release of epinephrine (A) and norepinephrine (NA) in NRO3Omin and vPAG under continuous electrical stimulation were observed by push-pull perfusion combined with high performance liquid chromatography-electrochemical detection. Results: After stimulated with NRO for 10-30 min, the content of A in vPAG perfusate increased significantly (P <0.05), and the content of NA in vPAG also increased after stimulating NRO for 10-20 min (P <0.05). On the basis of the above, ketamine (10μg / 10μl), a 5-HT2 receptor antagonist, was injected into the bilateral lateral ventricle before stimulation of NRO. There was no significant change in the content of A and NA in the NRO 10 ~ 30min after electrical stimulation ), Ketanserin blocked the effect of stimulating NRO-induced increases in A, NA release. CONCLUSION: The neurotransmitter of NRO to vPAG is ANA. The feedback effect of 5-HT neurons on NRO to vPAG may be mediated by 5-HT2 receptors.