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健康成年小鼠按体重随机分成1个对照组及3个染毒组,每组10只,雌雄各半。染毒组小鼠以灌胃方式分别给予20、40、80 mg/kg剂量的乙体氯氰菊酯,以食用色拉油稀释受试物质,对照组给予等量色拉油。3 h后处死小鼠,采用分光光度法和RT-PCR法检测小鼠脑组织谷氨酰胺酶(phosphate-activated glutam inase,PAG)活性及其mRNA水平。结果80 mg/kg剂量组全部小鼠及40 mg/kg剂量组3只小鼠于染毒2 h后出现兴奋症状。小鼠脑组织PAG活性随染毒剂量的增加而递增,PAG mRNA水平则随染毒剂量的增加而逐渐降低,其中,80 mg/kg剂量组小鼠脑组织PAG活性及其mR-NA水平与对照组相比,差异有统计学意义。
Healthy adult mice were randomly divided into 1 control group and 3 exposure groups according to their body weight, with 10 in each group. The mice in the exposure group were administered griseoforms of 20, 40, and 80 mg / kg by gavage respectively. The test substance was diluted with edible salad oil and the control group was given the same amount of salad oil. After 3 h, the mice were killed and the activity and mRNA level of PAG in mouse brain were detected by spectrophotometry and RT-PCR. Results All mice in the 80 mg / kg dose group and 3 mice in the 40 mg / kg dose group developed excitability symptoms after 2 hours of exposure. PAG activity in mice brain tissue increased with the dose increased, PAG mRNA levels decreased with the increase of the dose, in which, 80 mg / kg dose of brain tissue PAG activity and mR-NA levels and Compared with the control group, the difference was statistically significant.