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目的探讨对氨基水杨酸钠(PAS-Na)对亚急性染锰大鼠学习记忆及海马胶质原纤维酸性蛋白(GFAP)阳性细胞表达的影响。方法雄性SD大鼠40只随机分为正常对照组(对照组)(染锰组)低和高剂量PAS-Na治疗组(L-、H-PAS组)。染锰和PAS治疗大鼠每天腹腔注射(ip)MnCl2·4H2O 15 mg/kg,对照组大鼠ip等容量生理盐水,每周5 d,共3周。接着,PAS治疗大鼠每天背部皮下注射(sc)PAS-Na100或200 mg/kg,其余大鼠背部sc等容量生理盐水,连续5周。用Morris水迷宫检测大鼠学习记忆能力,免疫组织化学方法检测海马CA1区和齿状回GFAP阳性细胞数。结果染锰末周大鼠体重增加明显减低,PAS-Na治疗使染锰大鼠体重明显增加,以高剂量PAS-Na治疗组作用较为明显,差异有统计学意义(P<0.05或P<0.01)。染锰组逃避潜伏期、游泳总路程均比对照组长,H-PAS组逃避潜伏期、游泳总路程比染锰组缩短,差异有统计学意义(P<0.05)。染锰组GFAP阳性细胞数比对照组多,H-PAS组GFAP阳性细胞数较染锰组减少,差异有统计学意义(P<0.05或P<0.01)。结论 PAS-Na对锰致大鼠生长发育迟缓、学习记忆障碍和海马胶质细胞异常增生可能有干预作用。
Objective To investigate the effect of sodium salicylate (PAS-Na) on the learning and memory and the expression of glial fibrillary acidic protein (GFAP) in hippocampus of sub-acutely infected rats. Methods Forty male Sprague-Dawley rats were randomly divided into normal control group (control group) and manganese group (low-dose and high-dose PAS-Na treatment group (L-, H-PAS group. Rats exposed to manganese and PAS were intraperitoneally injected with MnCl2.4H2O 15 mg / kg ip and rats in the control group ip for 5 days a week for 3 weeks. PAS-treated rats were then injected subcutaneously (sc) PAS-Na100 or 200 mg / kg into the back of the rats, and the rest of the rats were sc sc-normal saline for 5 weeks. The learning and memory abilities of rats were detected by Morris water maze and the numbers of GFAP positive cells in hippocampal CA1 and dentate gyrus were detected by immunohistochemistry. Results Body weight gain was significantly reduced in rats exposed to manganese at the end of the experiment. The body weight of rats with Mn-Zn significantly increased after treatment with PAS-Na, and the effect was significant at the high dose of PAS-Na, with significant difference (P <0.05 or P <0.01) ). Manganese group escape latency, swimming total distance than the control group leader, H-PAS group escape latency, swimming total distance shorter than the manganese group, the difference was statistically significant (P <0.05). Compared with the control group, the number of GFAP positive cells in the Mn-Mn-Mn-Zn group was significantly decreased (P <0.05 or P <0.01). Conclusions PAS-Na may have an effect on the growth retardation, learning and memory impairment and abnormal proliferation of hippocampal astrocytes in rats induced by manganese.