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本文用荧光标记的受体底物〔Ｇｎβ１－２Ｍα１－６（Ｇｎβ１－２Ｍα１－３）Ｍβ１－４Ｇｎβ１－４（Ｆｕｃα１－６）Ｇｎ－ＰＡ〕，结合高效液相层析（ＨＰＬＣ）建立了细胞表面β１－４半乳糖基转移酶的活性检测方法。用这种方法研究ＨＬ６０细胞，发现不同的培养时间，其细胞表面的酶变化明显，在２４小时酶活性最高，此时细胞处在分裂间期。用佛波酯（ＰＭＡ），视黄酸（ＲＡ）等细胞诱导分化剂处理ＨＬ６０细胞株时，发现其表面活性发生了明显的变化，ＰＭＡ诱导的细胞其表面酶活性在２４小时变化最大，升高到对照的１．３倍；而ＲＡ处理的细胞其表面酶活性在７２小时变化最大，升高到对照的１．７２倍。
In this study, the cell surface was established by fluorescence-labeled receptor substrate [Gnβ1-2Mα1-6 (Gnβ1-2Mα1-3) Mβ1-4Gnβ1-4 (Fucα1-6) Gn-PA] combined with high performance liquid chromatography β1-4 galactosyltransferase activity assay. Using this method to study HL60 cells, found that different culture time, the cell surface enzyme changes significantly in 24 hours, the highest enzyme activity, the cells in the interphase. HL60 cells were treated with PMA and RA, and the surface activity of HL60 cells was significantly changed. The surface enzyme activity of PMA-induced cells changed most greatly in 24 hours, 1.3 times higher than that of the control, while the surface enzyme activities of RA-treated cells changed the most at 72 hours, reaching 1.72 times of the control.