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目的探讨低氧诱导因子-1α(HIF-1α)、诱导型一氧化氮合酶(iNOS)在溃疡性结肠炎(UC)组织中的表达及与疾病活动性的关系。方法采用免疫组织化学方法检测51例UC患者(活动期38例、缓解期13例)和20例正常对照组中的HIF-1α、iNOS表达。结果UC患者活动期HIF-1α表达显著高于正常人,差异有显著性(153.29±15.26vs193.28±16.65,P<0.01),缓解期HIF-1α表达与正常人差异无显著性(185.45±12.08vs193.28±16.65,P>0.05),HIF-1α的表达与Walmsley评分标准呈正相关;iNOS在溃疡性结肠炎患者活动期的表达显著高于正常人,差异有显著性(151.32±14.62vs196.67±17.43,P<0.01),缓解期的表达与正常人差异无显著性(189.93±15.20vs196.67±17.43,P>0.05),iNOS的表达与Walmsley评分标准也呈正相关;HIF-1α和iNOS在溃疡性结肠炎中表达水平呈正相关(r=0.627,P<0.05)。结论HIF-1α和iNOS均参与了溃疡性结肠炎的发病且与疾病的活动性有关,HIF-1α作为一种转录因子可能是iNOS基因表达中的一个重要调节者。
Objective To investigate the expression of hypoxia inducible factor-1α (HIF-1α) and inducible nitric oxide synthase (iNOS) in ulcerative colitis (UC) and its relationship with disease activity. Methods Immunohistochemistry was used to detect the expression of HIF-1α and iNOS in 51 patients with UC (38 active, 13 remission) and 20 normal controls. Results The expression of HIF-1α in active stage of UC patients was significantly higher than that of normal subjects (153.29 ± 15.26 vs 193.28 ± 16.65, P <0.01), while there was no significant difference between normal subjects and remission stage (185.45 ± 12.08vs193.28 ± 16.65, P> 0.05). The expression of HIF-1α was positively correlated with the Walmsley score. The expression of iNOS in patients with ulcerative colitis was significantly higher than that in normal controls (151.32 ± 14.62 vs 196 .67 ± 17.43, P <0.01). The expression of iNOS was positively correlated with the Walmsley score in the remission stage (189.93 ± 15.20 vs 196.67 ± 17.43, P> 0.05). The expression of HIF-1α And iNOS expression in ulcerative colitis was positively correlated (r = 0.627, P <0.05). Conclusion Both HIF-1α and iNOS are involved in the pathogenesis of ulcerative colitis. HIF-1α as a transcription factor may be an important regulator of iNOS gene expression.