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目的 研究肝病患者庚型肝炎病毒感染状况。方法 用酶链免疫吸附试验 (ELISA)检测 15 4例门诊和住院肝病患者 (均无输血史 )的抗 HGV ,15 4例中有 5 4例同时采用逆转录聚合酶链反应 (RT PCR)检测HGVRNA ,分别计算抗 HGV和HGVRNA的阳性率。以HGVRNA阳性为HGV感染的判断标准 ,来分析各类肝病患者HGV感染状况。结果 15 4例肝病患者抗 HGV阳性者 31例 ,阳性率 2 0 13% ;5 4例检测HGVRNA者阳性 13例 ,阳性率 2 4 0 7%。慢性肝炎和脂肪肝患者HGVRNA的阳性率 (比 )分别为 2 0 0 0 % (7/35 )和 5 /6。结论 (1)HGV感染在非经血途径传播的散发性肝炎患者中 ,也有相当程度的流行 ;(2 )HGV是慢性肝炎的致病因子之一 ;(3)在脂肪肝患者中检出HGVRNA的临床意义有待进一步研究。
Objective To study the hepatitis G virus infection in patients with liver diseases. Methods Enzyme linked immunosorbent assay (ELISA) was used to detect anti-HGV in 15 4 outpatients and hospitalized patients with liver disease (all without transfusion history). 54 of 154 patients were detected by reverse transcription-polymerase chain reaction (RT PCR) HGVRNA, were calculated anti-HGV and HGVRNA positive rate. To HGVRNA positive for HGV infection criteria to analyze the various types of liver disease HGV infection. Results Among the 154 patients with liver disease, 31 were positive for anti-HGV, with a positive rate of 20.13%. Among 54 cases of HGV RNA positive, 13 were positive, with a positive rate of 240.7%. The positive rates of HGVRNA in patients with chronic hepatitis and fatty liver were 200% (7/35) and 5/6 respectively. Conclusions (1) HGV infection is also endemic in patients with sporadic hepatitis that is not transmitted through the bloodstream; (2) HGV is one of the causative agents of chronic hepatitis; (3) HGVRNA is detected in patients with fatty liver Clinical significance needs further study.