论文部分内容阅读
本文介绍用普通SPA菌体试剂检测人B淋巴细胞SIgM和SIgG的方法(简称SPA花环试验)。对SPA花环形成的最适条件、重复性、特异性进行探讨,并用此法检测正常的人外周血、某些淋巴组织和淋巴细胞增殖性疾病患者的外周血及病变淋巴结中的B淋巴细胞,并将SPA花环试验与常规的免疫荧光法进行了比较。 用兔抗人IgM和IgG抗血清标记10％SPA菌体使成兔抗人IgM或IgG-SPA菌体试剂。取该试剂与外周血和淋巴组织中分离的淋巴细胞结合、洗涤后作湿片在油镜下观察,凡淋巴细胞外周有满圈或20个以上菌体吸附者为阳性,至少计数200
This article describes the use of ordinary SPA bacterial agent detection of human B lymphocyte SIgM and SIgG method (SPA spine trial). The optimal conditions, repeatability and specificity of the formation of SPA rosette were discussed, and the detection of B lymphocytes in peripheral blood of normal people, lymphoid tissue and lymphoproliferative diseases and the diseased lymph nodes by this method, The SPA garland test was compared with the conventional immunofluorescence method. Rabbit anti-human IgM or IgG-SPA bacterial cell reagent was labeled with rabbit anti-human IgM and IgG antiserum labeled with 10% SPA. Take the reagent and peripheral blood and lymphoid tissue lymphocytes isolated from the combination of wet after washing for observation under an oil microscope, where the lymphocytes are full circle around the circumference or more than 20 cell adsorbate positive, at least count 200