论文部分内容阅读
本文报告了在体外应用各种合成培养基保存和培养大恶丝虫微丝蚴的结果。所用的不同培养基有:(1)Tobies培养基(双相性的);(2)含胎牛血清的洛克氏液以及(3)含胎牛血清的RPMI1640组织培养基。这些培养基的pH调整到7.2～7.4,每ml培养基加200青霉素和100μg链霉素。
This paper reports the results of in vitro preservation and culture of a large filamentous microfilaria using a variety of synthetic media. The different media used were: (1) Tobies medium (biphasic); (2) Locke’s solution with fetal bovine serum; and (3) RPMI 1640 tissue culture medium with fetal calf serum. The pH of these media was adjusted to 7.2 to 7.4 with 200 penicillin and 100 μg streptomycin per ml of media.