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目的了解肿瘤侵袭率与ＰＧＩ２／ＴｘＡ２和ｔＰＡ／ＰＡＩ平衡的关系。方法参照Ｎｉｃｏｌｓｏｎ报道的方法建立体外黑色素瘤Ｂ１６侵袭血管内皮细胞单层模型，利用放射免疫酶联技术检测肿瘤侵袭过程中，ＰＧＩ２、ＴｘＡ２、ｔＰＡ、ＰＡＩ时相性分泌变化，以探究ＰＧＩ２／ＴｘＡ２、ｔＰＡ／ＰＡＩ比值变化，在肿瘤侵袭和转移过程中的作用。结果在肿瘤侵袭过程中，总ＰＧＩ２、ＰＡＩ分泌量呈下降趋势，ＴｘＡ２和ＰＡＩ分泌呈上升趋势；黑色素瘤Ｂ１６细胞能够通过分泌ｔＰＡ、ＰＡＩ、ＰＧＩ２和ＴｘＡ２来破坏血液循环系统中ＰＧＩ２／ＴｘＡ２和ｔＰＡ／ＰＡＩ平衡；ＰＧＩ２／ＴｘＡ２、ｔＰＡ／ＰＡＩ比值变化，肿瘤侵袭率呈对应性变动。结论ＰＧＩ２／ＴｘＡ２和ｔＰＡ／ＰＡＩ分泌的平衡失调可能是肿瘤侵袭和转移形成的重要原因之一。
Objective To understand the relationship between tumor invasion rate and the balance of PGI2/TxA2 and tPA/PAI. Methods According to the method reported by Nicolson, a single layer model of invasive melanoma B16 invading vascular endothelial cells was established. The phase changes of PGI2, TxA2, tPA, and PAI during tumor invasion were detected by using radioimmunoassay to explore PGI2/TxA2, tPA. The ratio of PAI/PAI changes during tumor invasion and metastasis. Results During tumor invasion, the secretion of total PGI2 and PAI showed a downward trend, TxA2 and PAI secretion increased. Melanoma B16 cells could secrete tPA, PAI, PGI2 and TxA2 to destroy PGI2/TxA2 and tPA in blood circulation system. /PAI balance; PGI2/TxA2, tPA/PAI ratio change, tumor invasion rate showed a corresponding change. Conclusion The balance of PGI2/TxA2 and tPA/PAI secretion may be one of the important causes of tumor invasion and metastasis.